Small scale protein purification column fabrication

In my study, sometimes I need small scale protein purification (some protein need be fresh purified each time). So I was looking for small protein purification column. Later, I found I can make small column for protein purification from 1250 microliter filter tip (Brand: Denville sharp).

First, use something clean and blunt to push the filter down.

 

Then, remove the rest of tip (lower part). 

 

Load resin (200 microliter) for protein purification.

The volume above resin is more than 500 microliter, I usually let wash buffer flow through by gravity. After wash 3-5 times, the result usually acceptable.

 

 

By the way, later our lab switch filter tips to AnantGuard Barrier tips from Midsci, the tip looks same and works fine. But later in 2013 the company seems change the barrier/filter to higher density stuff so the flow become very slow, I am not sure if denville tip changed or not.