昨天本來要做個 Western Blot, 於是開始找玻璃板來做gel.
結果好死不死本實驗室做 SDS gel 的板子很多都破了不能用,
由於這套系統的原產地是日本,所以要買新的板子還要跟日本訂,運過來還要付運費......
加上買新的還是有可能打破,那就自己用塑膠板切一個吧XD.
Yesterday I wanted to do a Western blot, so I was looking for glass plates.
However, in our lab several SDS PAGE glass plates were broken.
Because this SDS PAGE system came from Japan, we need place order from Japan and plus shipping......
Besides, new glass plate still could be destroyed, so I decided make one for myself from plastic plate. XD.
於是直接拿舊的做模子,關鍵的點標一標,線畫一畫然後開始用鋸子切.So, I used old glass plate as template, mark essential point and draw lines, then cut the plate by saw.
切完收工,左邊是舊的,右邊是我切的......看起來OK吧.Finish, the one on left is old glass plate, the one on the right is I made......
Looks OK.
試跑看看......(剛好在純化蛋白,就拿來試一試)Test run my recent purified protein.
跑完之後染色看起來OK嘍.After electrophoresis stain the gel, Looks OK.
注意事項:由於塑膠製品比較軟,所以安裝gel時要小心,可能會有氣泡,如果直接在架子上做gel應該可以排除這個問題.
By the way, because the plastic is much softer than glass, bubble might form when you assemble the gel to the apparatus. It should not be problem if you fix the plates on apparatus then make the gel.
